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Thermithiobacillus is a genus of nonsporeforming, rod-shaped, Gram-negative bacteria. The name derives from the Latin thermae, for warm baths, and the Classical Greek θείος, theios for sulfur. The type species of this genus was previously assigned to the genus Thiobacillus, but it was reclassified on the basis of 16S rRNA analysis in 2000, creating this genus.<ref name=Kelly2000>Template:Cite journal</ref>

A phylogenetic analysis, using 98 protein families confirmed this reassignment.<ref name=Hudson2014>Template:Cite journal</ref> This chemolithoautotrophic genus is obligately aerobic and moderately thermophilic (43-45 °C).<ref name=Kelly2000/> The type species is Thermithiobacillus tepidarius.<ref>LPSN lpsn.dsmz.de</ref> A second species, Thermithiobacillus plumbiphilus, was published in 2016.<ref name= Wanatable2016>Template:Cite journal</ref>

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