Editing Affinity chromatography (section)

{{Short description|Purification technique for biomolecules}}
{{use dmy dates|date=December 2021}}
'''Affinity chromatography''' is a method of separating a [[biomolecule]] from a mixture, based on a highly specific [[molecular binding|macromolecular binding]] interaction between the biomolecule and another substance.  The specific type of binding interaction depends on the biomolecule of interest; [[antigen]] and [[antibody]], [[enzyme]] and [[substrate (biochemistry)|substrate]], [[biochemistry receptor|receptor]] and [[ligand (biochemistry)|ligand]], or [[protein]] and [[nucleic acid]]<ref>{{Cite journal|last1=Aizpurua-Olaizola|first1=Oier|last2=Sastre Torano|first2=Javier|last3=Pukin|first3=Aliaksei|last4=Fu|first4=Ou|last5=Boons|first5=Geert Jan|last6=de Jong|first6=Gerhardus J.|last7=Pieters|first7=Roland J.|date=January 2018|title=Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors|journal=Electrophoresis|language=en|volume=39|issue=2|pages=344–347|doi=10.1002/elps.201700207|pmid=28905402|hdl=1874/362143 |s2cid=33657660|hdl-access=free}}</ref> binding interactions are frequently exploited for isolation of various biomolecules.  Affinity chromatography is useful for its high [[selectivity (chromatography)|selectivity]] and [[resolution (chromatography)|resolution]] of separation,<ref>{{Cite book|title=Fundamental Laboratory Approaches for Biochemistry and Biotechnology|last1=Ninfa|first1=Alexander J.|last2=Ballou|first2=David P.|last3=Benore|first3=Marilee|publisher=Wiley|year=2009|isbn=9780470087664|edition=2nd|page=133}}</ref><ref>{{cite web |url=https://www.bio-rad.com/en-us/applications-technologies/introduction-affinity-chromatography?ID=MWHAVG4VY |title="Introduction to Affinity Chromatography" |author=<!--Not stated--> |date=2020-09-14 |website=bio-rad.com |publisher=Bio-Rad |access-date=2020-09-14 }}</ref> compared to other chromatographic methods.