Editing Metabolic engineering (section)

[[File:Computational design and evaluation of DNA circuits to achieve optimal performance.svg|thumb|Some of the steps in engineering a metabolic process]]

'''Metabolic engineering''' is the practice of optimizing [[genetics|genetic]] and regulatory processes within [[Cell (biology)|cells]] to increase the cell's production of a certain substance. These processes are chemical networks that use a series of biochemical reactions and [[enzymes]] that allow cells to convert raw materials into molecules necessary for the cell's survival. Metabolic engineering specifically seeks to mathematically model these networks, calculate a yield of useful products, and pin point parts of the network that constrain the production of these products.<ref>Yang, Y.T., Bennet, G. N., San, K.Y., (1998) ''Genetic and Metabolic Engineering'', Electronic Journal of Biotechnology, {{ISSN|0717-3458}}</ref> [[Genetic engineering]] techniques can then be used to modify the network in order to relieve these constraints. Once again this modified network can be modeled to calculate the new product yield.

The ultimate goal of metabolic engineering is to be able to use these organisms to produce valuable substances on an industrial scale in a cost-effective manner.  Current examples include producing [[beer]], [[wine]], [[cheese]], [[pharmaceuticals]], and other [[biotechnology]] products.<ref>{{Cite journal|last=N. Milne, P. Thomsen, N. Mølgaard Knudsen, P. Rubaszka, M. Kristensen, L. Borodina|date=2020-07-01|title=Metabolic engineering of Saccharomyces cerevisiae for the de novo production of psilocybin and related tryptamine derivatives|journal=Metabolic Engineering|language=en|volume=60|pages=25–36|doi=10.1016/j.ymben.2019.12.007|pmid=32224264|pmc=7232020 |issn=1096-7176|doi-access=free}}</ref> Another possible area of use is the development of oil crops whose composition has been modified to improve their nutritional value.<ref>{{Cite journal |last=Zhou |first=Xue-Rong |last2=Liu |first2=Qing |last3=Singh |first3=Surinder |date=2023-03-27 |title=Engineering Nutritionally Improved Edible Plant Oils |url=https://www.annualreviews.org/doi/10.1146/annurev-food-052720-104852 |journal=Annual Review of Food Science and Technology |language=en |volume=14 |issue=1 |pages=247–269 |doi=10.1146/annurev-food-052720-104852 |issn=1941-1413|doi-access=free }}</ref> Some of the common strategies used for metabolic engineering are (1) overexpressing the gene encoding the rate-limiting enzyme of the biosynthetic pathway, (2) blocking the competing metabolic pathways, (3) heterologous gene expression, and (4) enzyme engineering.<ref>Kulkarni R, 2016. [http://www.ias.ac.in/describe/article/reso/021/03/0233-0237 Metabolic Engineering: Biological Art of Producing Useful Chemicals]. Resonance, 21 (3), 233-237.</ref>

Since cells use these metabolic networks for their survival, changes can have drastic effects on the cells' viability. Therefore, trade-offs in metabolic engineering arise between the cells ability to produce the desired substance and its natural survival needs.  Therefore, instead of directly deleting and/or overexpressing the genes that encode for metabolic enzymes, the current focus is to target the regulatory networks in a cell to efficiently engineer the metabolism.<ref>Vemuri, G.M, Aristidou, A.A, (2005) ''Metabolic Engineering in the -omics Era: Elucidating and Modulating Regulatory Networks'', Microbial Mol Biology Review vol. 69: 197-216</ref>