Editing Microsome (section)

{{Short description|Cellular debris}}
In [[cell biology]], '''microsomes''' are heterogeneous [[Vesicle (biology and chemistry)|vesicle-like]] artifacts (~20-200&nbsp;nm diameter) re-formed from pieces of the [[endoplasmic reticulum]] (ER) when [[eukaryotic]] cells are broken-up in the [[laboratory]]; microsomes are not present in healthy, living cells.<ref name="Voet">{{cite book |last1= Voet |first1= Donald |last2= Voet |first2= Judith G. | name-list-style = vanc |title= Biochemistry |url= https://archive.org/details/biochemistry00voet_1 |url-access= registration |edition=3rd |year= 2004 |publisher= Wiley |isbn= 0-471-19350-X |page=[https://archive.org/details/biochemistry00voet_1/page/1309 1309]}}</ref>

Rough (containing [[ribosome]]s) and smooth (without ribosomes) microsomes are made from the endoplasmic reticulum through [[cell disruption]]. These microsomes have an inside that is exactly the same as the endoplasmic reticulum lumen. Both forms of microsomes can be purified by a process known as equilibrium density [[centrifugation]]. Rough and smooth microsomes do differ in their proteins and rough microsomes have shown occurrence of translation and translocation at the same time besides certain exceptions from proteins in yeast.