Editing Primer walking (section)

{{short description|DNA sequencing method}}
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'''Primer walking''' is a technique used to clone a gene (e.g., disease gene) from its known closest markers (e.g., known gene). As a result, it is employed in cloning and sequencing efforts in plants, fungi, and mammals with minor alterations. This technique, also known as "directed sequencing," employs a series of Sanger sequencing reactions to either confirm the reference sequence of a known plasmid or PCR product based on the reference sequence (sequence confirmation service) or to discover the unknown sequence of a full plasmid or PCR product by designing primers to sequence overlapping sections (sequence discovery service).<ref>{{Cite web|last=Company|first=Azenta Life Sciences|title=Primer Walking FAQ|url=https://web.genewiz.com/primer-walking-faq|access-date=2021-11-20|website=web.genewiz.com|language=en}}</ref>