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Lineweaver–Burk plot
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== Definitions == The Lineweaver–Burk plot derives from a transformation of the [[Michaelis–Menten equation]], :<math> v = \frac{V a}{K_\mathrm{m} + a} </math> in which the rate <math>v</math> is a function of the substrate concentration <math>a</math> and two parameters <math>V</math>, the '''limiting rate''', and <math>K_\mathrm{m}</math>, the '''[[Michaelis constant]]'''. Taking reciprocals of both sides of this equation it becomes as follows: :<math>\frac{1}{v} = \frac{1}{V} + \frac{K_\mathrm{m}}{V} \cdot \frac{1}{a}</math> Thus plotting <math>1/v</math> against <math>1/a</math> generates a straight line with ordinate (y) intercept <math>1/V</math>, abscissa (x) intercept <math>-1/K_\mathrm{m}</math> and slope <math>K_\mathrm{m}/V</math>.
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