Editing Reverse transcription polymerase chain reaction (section)

==Nomenclature==

The combined RT-PCR and qPCR technique has been described as quantitative RT-PCR<ref name="pmid12325527">{{cite book | author = Joyce C | chapter = Quantitative RT-PCR: A Review of Current Methodologies | title = RT-PCR Protocols | series = Methods Mol. Biol. | volume = 193 | pages = 83–92 | year = 2002 | pmid = 12325527 | doi = 10.1385/1-59259-283-X:083 | isbn = 978-1-59259-283-8 }}</ref> or real-time RT-PCR<ref name="pmid20515509">{{cite journal  |vauthors=Kang XP, Jiang T, Li YQ, etal |title=A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus |journal=Virol. J. |volume=7 |page=113 |year=2010 |pmid=20515509 |pmc=2892456 |doi=10.1186/1743-422X-7-113 |doi-access=free }}</ref> (sometimes even called quantitative real-time RT-PCR<ref name="pmid15956331">{{cite journal |vauthors=Bustin SA, Benes V, Nolan T, Pfaffl MW |title=Quantitative real-time RT-PCR--a perspective |journal=J. Mol. Endocrinol. |volume=34 |issue=3 |pages=597–601 |date=June 2005 |pmid=15956331 |doi=10.1677/jme.1.01755 |citeseerx=10.1.1.528.6638 |s2cid=1754364 }}</ref>),  has been variously abbreviated as qRT-PCR,<ref name="pmid20204872">{{cite book |vauthors=Varkonyi-Gasic E, Hellens RP |title=Plant Epigenetics |chapter=qRT-PCR of Small RNAs |volume=631 |pages=109–22 |year=2010 |pmid=20204872 |doi=10.1007/978-1-60761-646-7_10 |series=Methods in Molecular Biology |isbn=978-1-60761-645-0 }}</ref> RT-qPCR,<ref name="pmid20215014">{{cite journal |vauthors=Taylor S, Wakem M, Dijkman G, Alsarraj M, Nguyen M |title=A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines |journal=Methods |volume=50 |issue=4 |pages=S1–5 |date=April 2010 |pmid=20215014 |doi=10.1016/j.ymeth.2010.01.005 }}</ref> RRT-PCR,<ref name="pmid12202562">
{{cite journal
 | vauthors=Spackman E, Senne DA, Myers TJ, et al
 | title=Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes
 | journal=J. Clin. Microbiol.
 | volume=40 | issue=9 | pages=3256–60
 | date=September 2002
 | pmid=12202562 | pmc=130722 | doi= 10.1128/jcm.40.9.3256-3260.2002
}}</ref> and rRT-PCR.<ref>
{{Cite web
 |url=https://www.fda.gov/media/136151/download|archive-url=https://web.archive.org/web/20200321220417/https://www.fda.gov/media/136151/download|url-status=dead|archive-date=March 21, 2020|title=ACCELERATED EMERGENCY USE AUTHORIZATION (EUA) SUMMARY COVID-19 RT-PCR TEST (LABORATORY CORPORATION OF AMERICA)
 |website=FDA
 |access-date=3 April 2020
}}</ref> In order to avoid confusion, the following abbreviations will be used consistently throughout this article:

{| class="wikitable"
|-
! Technique !! Abbreviation
|-
| Polymerase chain reaction || PCR
|-
| Reverse transcription polymerase chain reaction || RT-PCR
|-
| Real-time polymerase chain reaction || qPCR
|-
| RT-PCR / qPCR combined technique || qRT-PCR

|}
Not all authors, especially earlier ones, use this convention and the reader should be cautious when following links.  RT-PCR has been used to indicate both real-time PCR (qPCR) and reverse transcription PCR (RT-PCR).