Editing Trans-splicing (section)

== Genic ''trans''-splicing ==
Whereas "normal" [[splicing (genetics)|(''cis''-)splicing]] processes a single molecule, ''trans''-splicing generates a single RNA transcript from multiple separate [[pre-mRNA]]s. This phenomenon can be exploited for molecular therapy to address mutated gene products.<ref>{{cite journal | vauthors = Iwasaki R, Kiuchi H, Ihara M, Mori T, Kawakami M, Ueda H | title = Trans-splicing as a novel method to rapidly produce antibody fusion proteins | journal = Biochemical and Biophysical Research Communications | volume = 384 | issue = 3 | pages = 316–21 | date = July 2009 | pmid = 19409879 | doi = 10.1016/j.bbrc.2009.04.122 | url = https://zenodo.org/record/895586 }}</ref> Genic trans-splicing allows variability in RNA diversity and increases proteome complexity.<ref>{{Cite journal|last1=Lasda|first1=Erika L.|last2=Blumenthal|first2=Thomas|date=2011|title=Trans-splicing|url=https://onlinelibrary.wiley.com/doi/abs/10.1002/wrna.71|journal=WIREs RNA|language=en|volume=2|issue=3|pages=417–434|doi=10.1002/wrna.71|pmid=21957027|s2cid=209567118|issn=1757-7012|url-access=subscription}}</ref>

=== Oncogenesis ===
While some fusion transcripts occur via ''trans''-splicing in normal human cells,<ref name="Lei2016"/> ''trans''-splicing can also be the mechanism behind certain oncogenic [[fusion transcript]]s.<ref>{{cite journal | vauthors = Li H, Wang J, Mor G, Sklar J | title = A neoplastic gene fusion mimics trans-splicing of RNAs in normal human cells | journal = Science | volume = 321 | issue = 5894 | pages = 1357–61 | date = September 2008 | pmid = 18772439 | doi = 10.1126/science.1156725 | bibcode = 2008Sci...321.1357L | s2cid = 13605087 }}</ref><ref>{{cite journal | vauthors = Rickman DS, Pflueger D, Moss B, VanDoren VE, Chen CX, de la Taille A, Kuefer R, Tewari AK, Setlur SR, Demichelis F, Rubin MA | display-authors = 6 | title = SLC45A3-ELK4 is a novel and frequent erythroblast transformation-specific fusion transcript in prostate cancer | journal = Cancer Research | volume = 69 | issue = 7 | pages = 2734–8 | date = April 2009 | pmid = 19293179 | pmc = 4063441 | doi = 10.1158/0008-5472.CAN-08-4926 }}</ref>