Editing Affinity chromatography (section)

==== Alkaline phosphatase ====
[[Alkaline phosphatase]] from E. coli can be purified using a DEAE-Cellulose matrix. A. phosphatase has a slight negative charge, allowing it to weakly bind to the positively charged amine groups in the matrix. The enzyme can then be eluted out by adding buffer with higher salt concentrations.<ref>{{Cite book|title=Fundamental laboratory approaches for biochemistry and biotechnology|last1=Ninfa|first1=Alexander J.|last2=Ballou|first2=David P.|last3=Benore|first3=Marilee|date=2010|publisher=John Wiley|isbn=9780470087664|edition=2nd|location=Hoboken, N.J.|page=240|oclc=420027217}}</ref>