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==Assay types based on the targets being measured== ===DNA=== Assays for studying [[Protein–protein interaction|interactions]] of [[proteins]] with [[DNA]] include: *[[DNase footprinting assay]] *[[Filter binding assay]] *[[Gel shift assay]] ===Protein=== *[[Bicinchoninic acid assay]] (BCA assay) *[[Bradford protein assay]] *[[Lowry protein assay]]<ref>{{cite journal |vauthors=Lowry OH, Rosebrough NJ, Farr AL, Randall RJ |title=Protein measurement with the Folin phenol reagent |journal=J. Biol. Chem. |volume=193 |issue=1 |pages=265–75 |date=November 1951 |doi=10.1016/S0021-9258(19)52451-6 |pmid=14907713 |url=http://www.jbc.org/cgi/pmidlookup?view=long&pmid=14907713|doi-access=free }}</ref> *[[Secretion assay]] ===RNA=== *[[Nuclear run-on]] *[[Ribosome profiling]] ===Cell counting, viability, proliferation or cytotoxicity assays=== A cell-counting assay may determine the number of living cells, the number of dead cells, or the ratio of one cell type to another, such as enumerating and typing [[red blood cell|red]] versus different types of [[white blood cell|white]] blood cells. This is measured by different physical methods (light transmission, electric current change). But other methods use biochemical probing cell structure or physiology (stains). Another application is to monitor [[cell culture]] ([[Cell culture assays|assays]] of cell proliferation or [[Cytotoxicity#Measuring cytotoxicity|cytotoxicity]]). A cytotoxicity assay measures how toxic a chemical compound is to cells. *[[MTT assay]] *Cell Counting Kit-8 (WST-8 based cell viability assay) *SRB ([[Sulforhodamine B]]) assay *CellTiter-Glo® Luminescent Cell Viability Assay *Cell counting instruments and methods: [[CASY cell counting technology]], [[Coulter counter]], [[Electric cell-substrate impedance sensing]] *Cell [[viability assay]]s: [[Resazurin#Cell viability applications|resazurin method]], [[ATP test]], [[Ethidium homodimer assay]] (detect dead or dying cells), [[Bacteriological water analysis]], [[Clonogenic assay]]s, ... ===Environmental or food contaminants=== *[[Bisphenol F#Environmental contamination|Bisphenol F]] *[[Aquatic toxicology#Aquatic toxicity tests|Aquatic toxicity tests]] ===Surfactants=== *An [[MBAS assay]] indicates [[Ion#Anions and cations|anionic]] [[surfactant]]s in water with a bluing reaction. === Other cell assays === Many cell assays have been developed to assess specific parameters or response of cells ([[biomarker]]s, cell physiology). [[Cell biology#Techniques|Techniques used to study cells]] include : *reporter assays using i.e. [[Luciferase]], calcium signaling assays using [[Coelenterazine]], [[Carboxyfluorescein succinimidyl ester|CFSE]] or [[Calcein]] *[[Immunostaining]] of cells on slides by [[Microscopy]] ([[Immunohistochemical staining|ImmunoHistoChemistry or Fluorescence]]), on [[microplate]]s by [[Photometry (optics)|photometry]] including the [[ELISpot]] (and its variant [[ELISpot|FluoroSpot]]) to enumerate B-Cells or antigen-specific cells, in solution by [[Flow cytometry]] *[[Molecular biology]] techniques such as [[DNA microarray]]s, [[in situ hybridization]], combined to [[Polymerase chain reaction|PCR]], [[Computational genomics]], and [[Transfection]]; [[Cell fractionation]] or [[Immunoprecipitation]] *[[Migration assay]]s, [[Chemotaxis assay]] *[[Secretion assay]]s *[[Apoptosis]] assays such as the [[DNA laddering]] assay, the [[Nicoletti assay]], [[caspase]] activity assays, and [[Annexin V]] staining *[[Chemosensitivity assay]] measures the number of tumor cells that are killed by a cancer drug *[[Tetramer assay]] detect the presence of antigen specific T-cells *[[Gentamicin protection assay]] or survival assay or invasion assay to assess ability of pathogens (bacteria) to invade eukaryotic cells * [[Metastasis Assay]] * [[Enhancer-FACS-seq]], the technique using a cell sorting process before DNA sequencing ===Petrochemistry=== *[[Crude oil assay]] ===Virology=== The [[HPCE-based viral titer assay]] uses a proprietary, high-performance [[capillary electrophoresis]] system to determine [[baculovirus]] [[titer]]. The [[Trofile assay]] is used to determine [[HIV tropism]]. The viral [[plaque assay]] is to calculate the number of [[virus]]es present in a sample. In this technique the number of [[viral plaque]]s formed by a viral inoculum is counted, from which the actual virus concentration can be determined. ===Cellular secretions=== A wide range of cellular secretions (say, a specific [[antibody]] or [[cytokine]]) can be detected using the [[ELISA]] technique. The number of cells which secrete those particular substances can be determined using a related technique, the [[ELISPOT]] assay. ===Drugs=== *[[Drug test|Testing for Illegal Drugs]] *[[Schild regression#Radioligand binding assays|Radioligand binding assay]]
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