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Cloning vector
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===Bacterial artificial chromosome=== Insert size of up to 350 kb can be cloned in [[bacterial artificial chromosome]] (BAC). BACs are maintained in ''E. coli'' with a copy number of only 1 per cell.<ref name = "Casali_2003" /> BACs are based on [[Fertility factor (bacteria)|F plasmid]], another artificial chromosome called the [[P1-derived artificial chromosome|PAC]] is based on the [[P1 phage]].
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