Editing Polyphenol (section)

====Analysis techniques====
[[File:RP HPLC.PNG|thumb|right|Reversed-phase HPLC plot of separation of phenolic compounds. Smaller [[natural phenol]]s formed individual peaks while [[tannin]]s form a ''hump''.]]

[[Phosphomolybdic acid]] is used as a reagent for staining phenolics in [[thin layer chromatography]]. Polyphenols can be studied by [[spectroscopy]], especially in the ultraviolet domain, by [[fractionation]] or [[paper chromatography]]. They can also be analysed by chemical characterisation.

[[Instrumental chemistry]] analyses include [[Separation process|separation]] by [[high performance liquid chromatography]] (HPLC), and especially by [[reversed-phase liquid chromatography]] (RPLC), can be coupled to [[mass spectrometry]].<ref name=":0" />

=====Microscopy analysis=====
The [[DMACA reagent]] is an histological dye specific to polyphenols used in microscopy analyses. The [[autofluorescence]] of polyphenols can also be used, especially for localisation of lignin and [[suberin]].  Where fluorescence of the molecules themselves is insufficient for visualization by light microscopy, DPBA (diphenylboric acid 2-aminoethyl ester, also referred to as Naturstoff reagent A) has traditionally been used, at least in [[Botany|plant science]], to enhance the fluorescence signal.<ref>{{cite journal | vauthors = Ferrara BT, Thompson EP | title = A method for visualizing fluorescence of flavonoid therapeutics in vivo in the model eukaryote Dictyostelium discoideum | journal = BioTechniques | volume = 66 | issue = 2 | pages = 65–71 | date = February 2019 | pmid = 30744410 | doi = 10.2144/btn-2018-0084 | type = Paper | doi-access = free }}{{open access}}</ref>