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Morphometrics
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===Bone histomorphometry=== Histomorphometry of bone involves obtaining a [[Trephine|bone biopsy specimen]] and processing of bone specimens in the laboratory, obtaining estimates of the proportional volumes and surfaces occupied by different components of bone. First the bone is broken down by baths in highly concentrated [[ethanol]] and [[acetone]]. The bone is then embedded and [[staining|stained]] so that it can be visualized/analyzed under a [[microscope]].<ref>{{cite journal |author=Revell PA |title=Histomorphometry of bone |journal=J. Clin. Pathol. |volume=36 |issue=12 |pages=1323β31 |date=December 1983 |pmid=6361070 |pmc=498562 |doi= 10.1136/jcp.36.12.1323}}</ref> Obtaining a bone biopsy is accomplished by using a bone biopsy trephine.<ref>{{cite journal |author= Hodgson SF |last2= Johnson |first2= KA |last3= Muhs |first3= JM |last4= Lufkin |first4= EG |last5= McCarthy |first5= JT |title=Outpatient percutaneous biopsy of the iliac crest: methods, morbidity, and patient acceptance |journal=Mayo Clin Proc |volume=61 |issue=1 |pages=28β33 |date=January 1986 |pmid= 3941566 |doi=10.1016/s0025-6196(12)61395-0}}</ref>
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