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Reverse transcriptase
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=== Molecular biology === {{further|Reverse transcription polymerase chain reaction}} Reverse transcriptase is commonly used in research to apply the [[polymerase chain reaction]] technique to [[RNA]] in a technique called [[reverse transcription polymerase chain reaction]] (RT-PCR). The classical [[polymerase chain reaction|PCR]] technique can be applied only to [[DNA]] strands, but, with the help of reverse transcriptase, RNA can be transcribed into DNA, thus making [[Polymerase chain reaction|PCR]] analysis of RNA molecules possible. Reverse transcriptase is used also to create [[cDNA library|cDNA libraries]] from [[mRNA]]. The commercial availability of reverse transcriptase greatly improved knowledge in the area of molecular biology, as, along with other [[enzymes]], it allowed scientists to clone, sequence, and characterise RNA.{{cn|date=November 2024}}
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