Editing Transdifferentiation (section)

===Evaluation===

When examining transdifferentiated cells, it is important to look for markers of the target cell type and the absence of donor cell markers which can be accomplished using green fluorescent protein or immunodetection. It is also important to examine the cell function, [[epigenome]], [[transcriptome]], and [[proteome]] profiles. Cells can also be evaluated based upon their ability to integrate into the corresponding tissue in vivo<ref name="ReferenceB"/> and functionally replace its natural counterpart. In one study, transdifferentiating tail-tip [[fibroblasts]] into hepatocyte-like cells using transcription factors [[GATA4|Gata4]], Hnf1α and [[FOXA3|Foxa3]], and inactivation of p19(Arf) restored hepatocyte-like liver functions in only half of the mice using survival as a means of evaluation.<ref>{{Cite journal 
| last1 = Huang | first1 = P. 
| last2 = He | first2 = Z. 
| last3 = Ji | first3 = S. 
| last4 = Sun | first4 = H. 
| last5 = Xiang | first5 = D. 
| last6 = Liu | first6 = C. 
| last7 = Hu | first7 = Y. 
| last8 = Wang | first8 = X. 
| last9 = Hui | first9 = L. 
| doi = 10.1038/nature10116 
| title = Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors 
| journal = Nature 
| volume = 475 
| issue = 7356 
| pages = 386–389 
| year = 2011 
| pmid = 21562492 
| s2cid = 1115749 
}}</ref>