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Imidazole
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==Coordination chemistry== {{main|Transition metal imidazole complex}} Imidazole and its derivatives have high affinity for metal cations. One of the applications of imidazole is in the purification of [[His-tag]]ged [[protein]]s in [[Affinity chromatography#Immobilized metal ion affinity chromatography|immobilised metal affinity chromatography]] (IMAC). Imidazole is used to elute tagged proteins bound to [[nickel]] [[ion]]s attached to the surface of beads in the [[chromatography]] column. An excess of imidazole is passed through the column, which displaces the His-tag from nickel coordination, freeing the His-tagged proteins.
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