Editing Affinity chromatography (section)

==Weak affinity chromatography==
'''Weak affinity chromatography'''<ref name="Zopf 1990">{{cite journal |last1=Zopf |first1=D. |last2=Ohlson |first2=S. |title=Weak-affinity chromatography |journal=Nature |date=July 1990 |volume=346 |issue=6279 |pages=87–88 |doi=10.1038/346087a0 |issn=0028-0836|bibcode=1990Natur.346...87Z|s2cid=4306269}}</ref> ('''WAC''') is an affinity chromatography technique for affinity screening in drug development.<ref name="Duong 2011">{{Cite journal | last1=Duong-Thi | first1=M. D. | last2=Meiby | first2=E. | last3=Bergström | first3=M. | last4=Fex | first4=T. | last5=Isaksson | first5=R. | last6=Ohlson | first6=S. | doi=10.1016/j.ab.2011.02.022 | title=Weak affinity chromatography as a new approach for fragment screening in drug discovery | journal=Analytical Biochemistry | volume=414 | issue=1 | pages=138–146 | year=2011 | pmid=21352794}}</ref><ref name="Meiby 2013">{{Cite journal | last1=Meiby | first1=E. | last2=Simmonite | first2=H. | last3=Le Strat | first3=L. | last4=Davis | first4=B. | last5=Matassova | first5=N. | last6=Moore | first6=J. D. | last7=Mrosek | first7=M. | last8=Murray | first8=J. | last9=Hubbard | first9=R. E. | doi=10.1021/ac400715t | last10=Ohlson | first10=S. | title=Fragment Screening by Weak Affinity Chromatography: Comparison with Established Techniques for Screening against HSP90 | journal=Analytical Chemistry | volume=85 | issue=14 | pages=6756–6766 | year=2013 | pmid=23806099}}</ref> WAC is an affinity-based [[Chromatography|liquid chromatographic]] technique that separates [[chemical compound]]s based on their different weak affinities to an immobilized target. The higher affinity a compound has towards the target, the longer it remains in the separation unit, and this will be expressed as a longer retention time. The affinity measure and ranking of affinity can be achieved by processing the obtained retention times of analyzed compounds. Affinity chromatography is part of a larger suite of techniques used in [[chemoproteomics]] based drug target identification.

The WAC technology is demonstrated against a number of different [[protein]] targets – [[protease]]s, [[kinase]]s, [[Chaperone (protein)|chaperones]] and [[protein–protein interaction]] (PPI) targets. WAC has been shown to be more effective than established methods for fragment based screening.<ref name="Meiby 2013" />