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Restriction enzyme
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== Examples == {{See also|List of restriction enzyme cutting sites}} Examples of restriction enzymes include:<ref name="pmid6243774">{{cite journal | vauthors = Roberts RJ | title = Restriction and modification enzymes and their recognition sequences | journal = Nucleic Acids Research | volume = 8 | issue = 1 | pages = r63βr80 | date = January 1980 | pmid = 6243774 | pmc = 327257 | doi = 10.1093/nar/8.1.197-d }}</ref> {| class="wikitable sortable" style="border:1px solid #aaa;" |- ! Enzyme !! Source !! Recognition Sequence !! Cut |- |[[EcoRI]]||''[[Escherichia coli]]'' | 5'GAATTC 3'CTTAAG | 5'---G AATTC---3' 3'---CTTAA G---5' |- |[[EcoRII]]||''[[Escherichia coli]]'' | 5'CCWGG 3'GGWCC | 5'--- CCWGG---3' 3'---GGWCC ---5' |- |[[BamHI]]||''[[Bacillus amyloliquefaciens]]'' | 5'GGATCC 3'CCTAGG | 5'---G GATCC---3' 3'---CCTAG G---5' |- |[[HindIII]]||''[[Haemophilus influenzae]]'' | 5'AAGCTT 3'TTCGAA | 5'---A AGCTT---3' 3'---TTCGA A---5' |- |[[TaqI]]||''[[Thermus aquaticus]]'' | 5'TCGA 3'AGCT | 5'---T CGA---3' 3'---AGC T---5' |- |[[NotI]]||''[[Nocardia otitidis]]'' | 5'GCGGCCGC 3'CGCCGGCG | 5'---GC GGCCGC---3' 3'---CGCCGG CG---5' |- |[[HinFI]]||''[[Haemophilus influenzae]]'' | 5'GANTC 3'CTNAG | 5'---G ANTC---3' 3'---CTNA G---5' |- |[[Sau3AI]]||''[[Staphylococcus aureus]]'' | 5'GATC 3'CTAG | 5'--- GATC---3' 3'---CTAG ---5' |- |[[PvuII|PvuII*]]||''[[Proteus vulgaris]]'' | 5'CAGCTG 3'GTCGAC | 5'---CAG CTG---3' 3'---GTC GAC---5' |- |[[SmaI|SmaI*]]||''[[Serratia marcescens]]'' | 5'CCCGGG 3'GGGCCC | 5'---CCC GGG---3' 3'---GGG CCC---5' |- |[[HaeIII|HaeIII*]]||''[[Haemophilus influenzae biogroup aegyptius|Haemophilus aegyptius]]'' | 5'GGCC 3'CCGG | 5'---GG CC---3' 3'---CC GG---5' |- |[[HgaI]]<ref name="pmid2835753">{{cite journal | vauthors = Roberts RJ | title = Restriction enzymes and their isoschizomers | journal = Nucleic Acids Research | volume = 16 Suppl | issue = Suppl | pages = r271-313 | year = 1988 | pmid = 2835753 | pmc = 340913 | doi = 10.1093/nar/16.suppl.r271 }}</ref>||''[[Haemophilus gallinarum]]'' | 5'GACGC 3'CTGCG | 5'---NN NN---3' 3'---NN NN---5' |- |[[AluI|AluI*]]||''[[Arthrobacter luteus]]'' | 5'AGCT 3'TCGA | 5'---AG CT---3' 3'---TC GA---5' |- |[[EcoRV|EcoRV*]]||''[[Escherichia coli]]'' | 5'GATATC 3'CTATAG | 5'---GAT ATC---3' 3'---CTA TAG---5' |- |[[EcoP15I]]||''[[Escherichia coli]]'' | 5'CAGCAGN<sub>25</sub>NN 3'GTCGTCN<sub>25</sub>NN | 5'---CAGCAGN<sub>25</sub> NN---3' 3'---GTCGTCN<sub>25</sub>NN ---5' |- |[[KpnI]]<ref name="isbn0-7167-4366-3">{{cite book | vauthors = Krieger M, Scott MP, Matsudaira PT, Lodish HF, Darnell JE, Zipursky L, Kaiser C, Berk A | title = Molecular Cell Biology | edition = 5th | publisher = W.H. Freeman and Company | location = New York | year = 2004 | isbn = 0-7167-4366-3 | url = https://archive.org/details/molecularcellbio00harv }}</ref>||''[[Klebsiella pneumoniae]]'' | 5'GGTACC 3'CCATGG | 5'---GGTAC C---3' 3'---C CATGG---5' |- |[[PstI]]<ref name="isbn0-7167-4366-3"/>||''[[Providencia stuartii]]'' | 5'CTGCAG 3'GACGTC | 5'---CTGCA G---3' 3'---G ACGTC---5' |- |[[SacI]]<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces achromogenes]]'' | 5'GAGCTC 3'CTCGAG | 5'---GAGCT C---3' 3'---C TCGAG---5' |- |[[SalI]]<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces albus]]'' | 5'GTCGAC 3'CAGCTG | 5'---G TCGAC---3' 3'---CAGCT G---5' |- |[[ScaI]]*<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces caespitosus]]'' | 5'AGTACT 3'TCATGA | 5'---AGT ACT---3' 3'---TCA TGA---5' |- |[[SpeI]]||''[[Sphaerotilus natans]]'' | 5'ACTAGT 3'TGATCA | 5'---A CTAGT---3' 3'---TGATC A---5' |- |[[SphI]]<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces phaeochromogenes]]'' | 5'GCATGC 3'CGTACG | 5'---GCATG C---3' 3'---C GTACG---5' |- |[[StuI]]*<ref name="Sigma_R8013">{{cite web | url = http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/R8013 | title = Stu I from Streptomyces tubercidicus |publisher = Sigma-Aldrich |access-date=2008-06-07}}</ref><ref name="pmid6260571">{{cite journal | vauthors = Shimotsu H, Takahashi H, Saito H | title = A new site-specific endonuclease StuI from Streptomyces tubercidicus | journal = Gene | volume = 11 | issue = 3β4 | pages = 219β25 | date = November 1980 | pmid = 6260571 | doi = 10.1016/0378-1119(80)90062-1 }}</ref>||''[[Streptomyces tubercidicus]]'' | 5'AGGCCT 3'TCCGGA | 5'---AGG CCT---3' 3'---TCC GGA---5' |- |[[XbaI]]<ref name="isbn0-7167-4366-3"/>||''[[Xanthomonas badrii]]'' | 5'TCTAGA 3'AGATCT | 5'---T CTAGA---3' 3'---AGATC T---5' |} Key:<br/> <nowiki>*</nowiki> = blunt ends<br/> N = C or G or T or A<br/> W = A or T
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