Open main menu
Home
Random
Recent changes
Special pages
Community portal
Preferences
About Wikipedia
Disclaimers
Incubator escapee wiki
Search
User menu
Talk
Dark mode
Contributions
Create account
Log in
Editing
Microscopy
(section)
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==== Single plane illumination microscopy and light sheet fluorescence microscopy ==== {{Main|Light sheet fluorescence microscopy}} Using a plane of light formed by focusing light through a cylindrical lens at a narrow angle or by scanning a line of light in a plane perpendicular to the axis of objective, high resolution optical sections can be taken.<ref>{{Cite journal| doi = 10.1016/S0378-5955(02)00493-8| pmid = 12204356| issn = 0378-5955| volume = 71| pages = 119β128| last = Voie| first = A.H.| title = Imaging the intact guinea pig tympanic bulla by orthogonal-plane fluorescence optical sectioning microscopy| journal = Hearing Research| year = 1993| issue = 1β2| s2cid = 12775304}}</ref><ref>{{Cite journal| doi = 10.1063/1.2428277| issn = 0034-6748| volume = 78| last = Greger| first = K.| author2 = J. Swoger| author3 = E. H. K. Stelzer| title = Basic building units and properties of a fluorescence single plane illumination microscope| journal = Review of Scientific Instruments| year = 2007| issue = 2| pages = 023705β023705β7| pmid = 17578115| bibcode = 2007RScI...78b3705G| doi-access = free}}</ref><ref>{{Cite journal| doi = 10.1155/2012/206238| pmid = 22567307| pmc = 3335623| issn = 2090-2743| volume = 2012| page = 206238| last = Buytaert| first = J.A.N. |author2=E. Descamps |author3=D. Adriaens |author4=J.J.J. Dirckx| title = The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens| journal = Anatomy Research International| year = 2012| arxiv = 1106.3162| doi-access = free}}</ref> Single plane illumination, or light sheet illumination, is also accomplished using [[Beam expander#Extra-cavity beam shaping|beam shaping techniques incorporating multiple-prism beam expanders]].<ref>F. J. Duarte, in ''High Power Dye Lasers'' (Springer-Verlag, Berlin,1991) Chapter 2.</ref><ref>[[F. J. Duarte|Duarte FJ]] (1993), Electro-optical interferometric microdensitometer system, [http://www.patentgenius.com/patent/5255069.html ''US Patent'' 5255069] {{Webarchive|url=https://web.archive.org/web/20171013042159/http://www.patentgenius.com/patent/5255069.html |date=2017-10-13 }}.</ref> The images are captured by CCDs. These variants allow very fast and high signal to noise ratio image capture.
Edit summary
(Briefly describe your changes)
By publishing changes, you agree to the
Terms of Use
, and you irrevocably agree to release your contribution under the
CC BY-SA 4.0 License
and the
GFDL
. You agree that a hyperlink or URL is sufficient attribution under the Creative Commons license.
Cancel
Editing help
(opens in new window)