Editing Point mutation (section)

===Use in molecular biology===

Because RIP is so efficient at detecting and mutating repeats, biologists working on ''[[Neurospora crassa]]'' have used it as a tool for [[mutagenesis]]. A second copy of a single-copy [[gene]] is first [[Transformation (genetics)|transformed]] into the [[genome]]. The fungus must then [[mating|mate]] and go through its sexual cycle to activate the RIP machinery. Many different mutations within the duplicated gene are obtained from even a single fertilization event so that inactivated alleles, usually due to [[nonsense mutations]], as well as alleles containing [[missense mutations]] can be obtained.<ref name="pmid2150906">{{cite journal| author=Selker EU| title=Premeiotic instability of repeated sequences in Neurospora crassa. | journal=Annu Rev Genet | year= 1990 | volume= 24 | pages= 579–613 | pmid=2150906 | doi=10.1146/annurev.ge.24.120190.003051 }}</ref>