Editing Coombs test (section)

==Enhancement media==
Both [[IgM]] and [[IgG]] [[antibodies]] bind strongly with their complementary antigens. [[IgG]] antibodies are most reactive at 37°C.  IgM antibodies are easily detected in [[saline (medicine)|saline]] at [[room temperature]] as IgM antibodies are able to bridge between RBC's owing to their large size, efficiently creating what is seen as [[agglutination (biology)|agglutination]].  IgG antibodies are smaller and require assistance to bridge well enough to form a visual [[agglutination (biology)|agglutination]] reaction.  Reagents used to enhance IgG detection are referred to as potentiators.  RBCs have a net negative charge called zeta potential which causes them to have a natural repulsion for one another. Potentiators reduce the zeta potential of RBC membranes. Common potentiators include low ionic strength solution (LISS), [[albumin]], [[polyethylene glycol]] (PEG), and proteolytic [[enzymes]].