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Plasmid
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=== Yeast plasmids === [[Yeast]]s naturally harbour various plasmids. Notable among them are 2 μm plasmids—small circular plasmids often used for [[genetic engineering]] of yeast—and linear pGKL plasmids from ''[[Kluyveromyces lactis]]'', that are responsible for [[killer phenotypes]].<ref>{{cite journal | vauthors = Gunge N, Murata K, Sakaguchi K | title = Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis | journal = Journal of Bacteriology | volume = 151 | issue = 1 | pages = 462–464 | date = July 1982 | pmid = 7045080 | pmc = 220260 | doi = 10.1128/JB.151.1.462-464.1982 }}</ref> Other types of plasmids are often related to yeast cloning vectors that include: * ''Yeast integrative plasmid (YIp)'', yeast vectors that rely on integration into the host chromosome for survival and replication, and are usually used when studying the functionality of a solo gene or when the gene is toxic. Also connected with the gene URA3, that codes an enzyme related to the biosynthesis of pyrimidine nucleotides (T, C); * ''Yeast Replicative Plasmid (YRp)'', which transport a sequence of chromosomal DNA that includes an origin of replication. These plasmids are less stable, as they can be lost during budding.
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