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Diffraction
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=== Diffraction before destruction === A new way to image single biological particles has emerged since the 2010s, utilising the bright X-rays generated by X-ray [[free-electron laser]]s. These femtosecond-duration pulses will allow for the (potential) imaging of single biological macromolecules. Due to these short pulses, radiation damage can be outrun, and diffraction patterns of single biological macromolecules will be able to be obtained.<ref>{{Cite journal|last1=Neutze|first1=Richard|last2=Wouts|first2=Remco|last3=van der Spoel|first3=David|last4=Weckert|first4=Edgar|last5=Hajdu|first5=Janos|date=August 2000|title=Potential for biomolecular imaging with femtosecond X-ray pulses|url=https://www.nature.com/articles/35021099|journal=Nature|language=en|volume=406|issue=6797|pages=752β757|doi=10.1038/35021099|pmid=10963603|bibcode=2000Natur.406..752N |s2cid=4300920|issn=1476-4687|url-access=subscription}}</ref><ref>{{Cite journal|last1=Chapman|first1=Henry N.|last2=Caleman|first2=Carl|last3=Timneanu|first3=Nicusor|date=2014-07-17|title=Diffraction before destruction|url= |journal=Philosophical Transactions of the Royal Society B: Biological Sciences|volume=369|issue=1647|pages=20130313|doi=10.1098/rstb.2013.0313|pmc=4052855|pmid=24914146}}</ref>
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