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Digital microfluidics
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==== Individual droplet addressability ==== DMF allows for droplet level addressability, meaning individual droplets can be treated as spatially distinct [[microreactor]]s.<ref name="Kothamachu_2020" /> This level of droplet control is important for workflows where reactions are sensitive to the order of reagent mixing and incubation times, but where the optimal values of these parameters may still need to be determined. These types of workflows are common in [[Cell-free system|cell-free biology]], and Liu et al. were able to demonstrate a proof-of-concept DMF-based strategy for carrying out remote-controlled [[Cell-free protein synthesis|cell-free protein expression]] on an OpenDrop chip.<ref name="Liu_2020">{{cite journal | vauthors = Liu D, Yang Z, Zhang L, Wei M, Lu Y | title = Cell-free biology using remote-controlled digital microfluidics for individual droplet control | journal = RSC Advances | volume = 10 | issue = 45 | pages = 26972β26981 | date = July 2020 | pmid = 35515808 | pmc = 9055536 | doi = 10.1039/D0RA04588H | bibcode = 2020RSCAd..1026972L }}</ref>
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