Editing Small interfering RNA (section)

=== Transfection ===
In this technique siRNA first must be designed against the target gene. Once the siRNA is configured against the gene it has to be effectively delivered through a transfection protocol. Delivery is usually done by [[cationic liposome]]s, polymer nanoparticles, and lipid conjugation.<ref>{{cite web|title=Transfection: ''In Vitro'' Transfection|first1=Alex|last1=Fanelli| name-list-style = vanc |url=http://transfection.ws/|date=2016|access-date=5 December 2017}}</ref> This method is advantageous because it can deliver siRNA to most types of cells, has high efficiency and reproducibility, and is offered commercially. The most common commercial reagents for [[transfection]] of siRNA are [[Lipofectamine]] and Neon Transfection. However, it is not compatible with all cell types and has low in vivo efficiency.<ref>{{cite journal | vauthors = Jensen K, Anderson JA, Glass EJ | title = Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation | journal = Veterinary Immunology and Immunopathology | volume = 158 | issue = 3–4 | pages = 224–32 | date = April 2014 | pmid = 24598124 | pmc = 3988888 | doi = 10.1016/j.vetimm.2014.02.002 }}</ref><ref>{{cite book|title=Textbook of Medical Biochemistry| vauthors = Chatterjea MN |location=New Delhi |publisher=Jaypee Brothers Medical Publishers|year=2012|pages=304|edition=8th}}</ref>