Editing Active site (section)

==Inhibitors==
{{Main|Enzyme inhibitor}}
Inhibitors disrupt the interaction between enzyme and substrate, slowing down the rate of a reaction. There are different types of inhibitor, including both reversible and irreversible forms.

[[Competitive inhibition|Competitive inhibitors]] are inhibitors that only target free enzyme molecules. They compete with substrates for free enzyme acceptor and can be overcome by increasing the substrate concentration. They have two mechanisms. Competitive inhibitors usually have structural similarities to the substrates and or ES complex. As a result, they can fit into the active site and trigger favourable interactions to fill in the space and block substrates from entry. They can also induce transient conformational changes in the active site so substrates cannot fit perfectly with it. After a short period of time, competitive inhibitors will drop off and leave the enzyme intact.

Inhibitors are classified as [[non-competitive inhibition|non-competitive inhibitor]]s when they bind both free enzyme and ES complex. Since they do not compete with substrates for the active site, they cannot be overcome by simply increasing the substrate concentration. They usually bind to a different site on the enzyme and alter the 3-dimensional structure of the active site to block substrates from entry or leaving the enzyme.

[[Enzyme inhibitor|Irreversible inhibitors]] are similar to competitive inhibitors as they both bind to the active site. However, irreversible inhibitors form irreversible covalent bonds with the amino acid residues in the active site and never leave. Therefore, the active site is occupied and the substrate cannot enter. Occasionally the inhibitor will leave but the catalytic site is permanently altered in shape. These inhibitors usually contain electrophilic groups like [[halogen]] substitutes and [[epoxides]]. As time goes by more and more enzymes are bound by irreversible inhibitors and cannot function anymore.

{| class="wikitable"
|-
!  !! Example !! Binds active site? !! Reduces rate of reaction?
|-
| '''Competitive reversible inhibitor''' || HIV protease inhibitors || Yes|| Yes
|-
| '''Non-competitive reversible inhibitor''' || Heavy metals such as lead and mercury || No || Yes
|-
| '''Irreversible inhibitor''' || Cyanide || Yes || Yes
|}