Editing Reverse transcription polymerase chain reaction (section)

==== Step two ====
Add master mix which contains buffer, dNTP mix, MgCl<sub>2</sub>, Taq polymerase, and nuclease-free water to each PCR tube. Then add the necessary primer to the tubes. Next, place the PCR tubes in a thermal cycler for 30 cycles of the amplification program. This includes denaturation, annealing, and elongation. The products of RT-PCR can be analyzed with gel electrophoresis.<ref>{{cite web|title=RT-PCR Two-Step Protocol|url=http://ocw.mit.edu/courses/biology/7-16-experimental-molecular-biology-biotechnology-ii-spring-2005/labs/rt_pcr_2step.pdf|publisher=MIT|access-date=12 December 2012}}</ref>