Editing DAPI (section)

==Modelling of absorption and fluorescence properties==
This DNA fluorescent probe has been effectively modeled<ref>{{cite journal|last1=Biancardi|first1=Alessandro|last2=Biver|first2=Tarita|last3=Secco|first3=Fernando|last4=Mennucci|first4=Benedetta|title=An investigation of the photophysical properties of minor groove bound and intercalated DAPI through quantum-mechanical and spectroscopic tools. |doi=10.1039/C3CP44058C  |pmid=23423468 |journal=Phys. Chem. Chem. Phys. |year=2013 |volume=15 |issue=13 |pages=4596–603 |bibcode=2013PCCP...15.4596B }}</ref> using the [[time-dependent density functional theory]], coupled with the IEF version of the [[polarizable continuum model]]. This quantum-mechanical modeling has rationalized the absorption and fluorescence behavior given by minor groove binding and [[intercalation (biochemistry)|intercalation]] in the DNA pocket, in term of a reduced structural flexibility and polarization.