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Protein sequencing
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=== Hydrolysis === [[Hydrolysis]] is done by heating a sample of the protein in 6 M [[hydrochloric acid]] to 100β110 Β°C for 24 hours or longer. Proteins with many bulky [[hydrophobic]] groups may require longer heating periods. However, these conditions are so vigorous that some amino acids ([[serine]], [[threonine]], [[tyrosine]], [[tryptophan]], [[glutamine]], and [[cysteine]]) are degraded. To circumvent this problem, Biochemistry Online suggests heating separate samples for different times, analysing each resulting solution, and extrapolating back to zero hydrolysis time. Rastall suggests a variety of reagents to prevent or reduce degradation, such as [[thiol]] [[reagent]]s or [[phenol]] to protect tryptophan and tyrosine from attack by chlorine, and pre-oxidising cysteine. He also suggests measuring the quantity of [[ammonia]] evolved to determine the extent of [[amide hydrolysis]].
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