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Pyrosequencing
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==Limitations== Currently, a limitation of the method is that the lengths of individual reads of DNA sequence are in the neighborhood of 300-500 nucleotides, shorter than the 800-1000 obtainable with [[DNA sequencing#Chain-termination methods|chain termination]] methods (e.g. Sanger sequencing). This can make the process of [[genome assembly]] more difficult, particularly for sequences containing a large amount of [[repetitive DNA]]. Lack of proof-reading activity limits accuracy of this method.
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