Editing Recalcitrant seed (section)

== Storage ==
Preservation of recalcitrant seeds remains experimental. Some approaches include:
* Removal of the embryo inside for [[cryopreservation]] (liquid nitrogen).<ref name="CPC"/>
* Cryopreservation of the whole seed in an anti-freezing solution.<ref name="freezing-nymph">{{Cite book |last=Lee |first=Chung-Hao |url=https://lch99310.github.io/files/Cryopreservation%20of%20seeds%20of%20blue%20waterlily%20-Nymphaea%20caerulea-%20using%20glutathione%20adding%20plant%20vitrification%20solution%2C%20PVS%2B.pdf |title=Cryopreservation of seeds of blue waterlily (Nymphaea caerulea) using glutathione adding plant vitrification solution, PVS+ / 埃及藍睡蓮種子的冷凍保存 — 使用添加穀胱甘肽的植物抗凍配方 |publisher=National Tsing Hua University |year=2016 |language=English}}</ref>

'''Intermediate seeds''' are between orthodox and recalcitrant seeds in their survivability. They are initially identified by their inability to survive conventional dry-freezing storage while being able to survive cryopreservation as a whole. The storage guideline is to put them in refrigeration at 45&ndash;65% RH, for a maximum of five years.<ref name="CPC">{{cite web |title=Orthodox, Intermediate, and Recalcitrant Seed |url=https://saveplants.org/best-practices/difference-between-orthodox-intermediate-and-recalcitrant-seed/ |website=Center for Plant Conservation}}</ref>