Editing Wobble base pair (section)

===Wobble hypothesis===
These notions led [[Francis Crick]] to the creation of the wobble hypothesis, a set of four relationships explaining these naturally occurring attributes.
# The first two bases in the codon create the coding specificity, for they form strong Watson-Crick base pairs and bond strongly to the anticodon of the tRNA.
# When reading [[Directionality (molecular biology)|5']] to [[Directionality (molecular biology)|3']] the first nucleotide in the anticodon (which is on the tRNA and pairs with the last nucleotide of the codon on the mRNA) determines how many nucleotides the tRNA actually distinguishes. <br />If the first nucleotide in the anticodon is a C or an A, pairing is specific and acknowledges original Watson-Crick pairing, that is: only one specific codon can be paired to that tRNA. If the first nucleotide is U or G, the pairing is less specific and in fact two bases can be interchangeably recognized by the tRNA. [[Inosine]] displays the true qualities of wobble, in that if that is the first nucleotide in the anticodon, any of three bases in the original codon can be matched with the tRNA.
# Due to the specificity inherent in the first two nucleotides of the codon, if one [[amino acid]] is coded for by multiple anticodons and those anticodons differ in either the second or third position (first or second position in the codon) then a different tRNA is required for that anticodon.
# The minimum requirement to satisfy all possible codons (61 excluding three stop codons) is 32 tRNAs. That is 31 tRNAs for the amino acids and one initiation codon.<ref>{{cite book|last1=Cox|first1=Michael M.|last2=Nelson|first2=David L.|title=Lehninger Principles of Biochemistry|date=2013|publisher=W.H. Freeman|location=New York|edition=6th|pages=[https://archive.org/details/lehningerprincip00lehn_1/page/1108 1108–1110]|chapter=Protein Metabolism: Wobble Allows Some tRNA's to Recognize More than One Codon|chapter-url=https://books.google.com/books?id=5Ek9J4p3NfkC&pg=PA1067|access-date=31 October 2015|isbn=9780716771081|url=https://archive.org/details/lehningerprincip00lehn_1/page/1108}} <!-- URL for 5th ed. --></ref>