Editing Protein design (section)

===Protein resurfacing===

Protein resurfacing consists of designing a protein's surface while preserving the overall fold, core, and boundary regions of the protein intact. Protein resurfacing is especially useful to alter the binding of a protein to other proteins. One of the most important applications of protein resurfacing was the design of the RSC3 probe to select broadly neutralizing HIV antibodies at the NIH Vaccine Research Center. First, residues outside of the binding interface between the gp120 HIV envelope protein and the formerly discovered b12-antibody were selected to be designed. Then, the sequence spaced was selected based on evolutionary information, solubility, similarity with the wild-type, and other considerations. Then the RosettaDesign software was used to find optimal sequences in the selected sequence space. RSC3 was later used to discover the broadly neutralizing antibody VRC01 in the serum of a long-term HIV-infected non-progressor individual.<ref>{{cite journal|last=Burton|first=DR|author2=Weiss, RA |title=AIDS/HIV. A boost for HIV vaccine design.|journal=Science|date=August 13, 2010|volume=329|issue=5993|pages=770–3|pmid=20705840|bibcode= 2010Sci...329..770B |doi= 10.1126/science.1194693|s2cid=206528638}}</ref>