Editing Candida albicans (section)

===Selection markers===
The selection markers most used in ''C. albicans'' are the CaNAT1 resistance marker (confers resistance against [[nourseothricin]]) and MPAr or IMH3r (confers resistance to [[mycophenolic acid]]).<ref>{{cite journal | vauthors = Shen J, Guo W, Köhler JR | title = CaNAT1, a heterologous dominant selectable marker for transformation of Candida albicans and other pathogenic Candida species | journal = Infection and Immunity | volume = 73 | issue = 2 | pages = 1239–1242 | date = February 2005 | pmid = 15664973 | pmc = 547112 | doi = 10.1128/IAI.73.2.1239-1242.2005 }}</ref>
Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The [[URA3]] marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of ''C. albicans''.<ref>{{cite journal | vauthors = Cheng S, Nguyen MH, Zhang Z, Jia H, Handfield M, Clancy CJ | title = Evaluation of the roles of four Candida albicans genes in virulence by using gene disruption strains that express URA3 from the native locus | journal = Infection and Immunity | volume = 71 | issue = 10 | pages = 6101–6103 | date = October 2003 | pmid = 14500538 | pmc = 201070 | doi = 10.1128/IAI.71.10.6101-6103.2003 }}</ref> Besides the URA3 selection one can also use the histidine, leucine and arginine autotrophy. The advantage of using those autotrophies lies in the fact that they exhibit wild-type or nearly wild-type virulence in a mouse model compared to the URA3 system.<ref>{{cite journal | vauthors = Noble SM, Johnson AD | title = Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans | journal = Eukaryotic Cell | volume = 4 | issue = 2 | pages = 298–309 | date = February 2005 | pmid = 15701792 | pmc = 549318 | doi = 10.1128/EC.4.2.298-309.2005 }}</ref> One application of the leucine, arginine and histidine autotrophy is for example the candida two-hybrid system.<ref name="stynen"/>