Editing Flow cytometry (section)

=== Measuring genome size ===
Flow cytometry has been used to measure [[genome size]]s, or more precisely: the amount of [[DNA]] in a [[Cell (biology)|cell]] or [[Cell nucleus|nucleus]]. Although genomes can be analyzed with more precision by [[Whole genome sequencing|genome sequencing]], this is often difficult due to a high fraction of [[Microchromosome|micro-chromosomes]] or [[Repeated sequence (DNA)|repetitive sequences]] which may be missed by sequencing (or which get filtered out during the analysis step when they cannot be assigned to [[chromosome]]s). However, flow cytometry is not perfect either. The resulting genome sizes may differ based on the dye used. An analysis of fish genomes resulted in significantly different genome sizes when [[propidium iodide]] (PI) and [[DAPI]] were used, respectively. For instance, the genome of ''[[Japanese eel|Anguilla japonica]]'' was found to contain 1.09 pg of DNA with PI vs. 1.25 pg with DAPI. Similarly, the genome of ''[[Chinese high-fin banded shark|Myxocyprinus asiaticus]]'' was found to contain 2.75 pg of DNA (PI) vs. 3.08 pg (DAPI). That is, the differences were on the order of 12–14%.<ref>{{Cite journal |last1=Zhu |first1=Dongmei |last2=Song |first2=Wen |last3=Yang |first3=Kun |last4=Cao |first4=Xiaojuan |last5=Gul |first5=Yasmeen |last6=Wang |first6=Weiming |date=2012 |title=Flow cytometric determination of genome size for eight commercially important fish species in China |url=https://www.jstor.org/stable/23279365 |journal=In Vitro Cellular & Developmental Biology. Animal |volume=48 |issue=8 |pages=507–517 |doi=10.1007/s11626-012-9543-7 |jstor=23279365 |pmid=22956044 |s2cid=255351169 |issn=1071-2690|url-access=subscription }}</ref>