Editing Protein engineering (section)

==== Focused mutagenesis ====
Focused mutagenic methods produce mutations at predetermined amino acid residues. These techniques require and understanding of the sequence-function relationship for the protein of interest. Understanding of this relationship allows for the identification of residues which are important in stability, stereoselectivity, and catalytic efficiency.<ref name=PoluriBook/>{{page needed|date=May 2017}} Examples of methods that produce focused mutagenesis are below.