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Adenosine diphosphate
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===Catabolism=== The ten-step [[catabolic]] pathway of [[glycolysis]] is the initial phase of free-energy release in the breakdown of [[glucose]] and can be split into two phases, the preparatory phase and payoff phase. ADP and [[phosphate]] are needed as precursors to synthesize ATP in the payoff reactions of the [[TCA cycle]] and [[oxidative phosphorylation]] mechanism.<ref>{{cite journal |vauthors =Jensen TE, Richter EA |title=Regulation of glucose and glycogen metabolism during and after exercise |journal=J. Physiol. |volume=590 |issue=Pt 5 |pages=1069β76 |date=March 2012|pmid=22199166 |pmc=3381815 |doi=10.1113/jphysiol.2011.224972 }}</ref> During the payoff phase of glycolysis, the enzymes phosphoglycerate kinase and pyruvate kinase facilitate the addition of a phosphate group to ADP by way of [[substrate-level phosphorylation]].<ref>{{cite journal |vauthors =Liapounova NA, Hampl V, Gordon PM, Sensen CW, Gedamu L, Dacks JB |title=Reconstructing the mosaic glycolytic pathway of the anaerobic eukaryote Monocercomonoides |journal=Eukaryotic Cell |volume=5 |issue=12 |pages=2138β46 |date=December 2006|pmid=17071828 |pmc=1694820 |doi=10.1128/EC.00258-06 }}</ref> [[File:Glycolysis overview.svg|thumb|Glycolysis overview]]
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