Editing Anaphase-promoting complex (section)

===CDC27/APC3===

One of the subunits that exhibit the TPR motif, CDC27 has been identified to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1, suggesting that it may have involvement in the timing of M phase.<ref>"Entrez Gene: CDC27 cell division cycle 27 homolog (S. cerevisiae)".</ref>  Evidence shows that CDC27 is involved in a ternary complex with SMAD2/3 and Cdh1, which is created in response to TGFβ signalling. Because of its interaction with Cdh1 in particular, it has a potential role in determining affinity between APC and its activators Cdc20 and Cdh1. A study suggests that TGF-β-induced Cdc27 phosphorylation enhances interaction between cdc27 and Cdh1–which is directly involved in activating APC.<ref>{{cite journal | vauthors = Zhang L, Fujita T, Wu G, Xiao X, Wan Y | title = Phosphorylation of the anaphase-promoting complex/Cdc27 is involved in TGF-beta signaling | journal = The Journal of Biological Chemistry | volume = 286 | issue = 12 | pages = 10041–50 | date = March 2011 | pmid = 21209074 | pmc = 3060455 | doi = 10.1074/jbc.M110.205518 | doi-access = free }}</ref> CDC27 can serve as  a vehicle through which TGFβ signalling can activate APC. Induced CDC27 hyperphosphorylation by TGFβ showed elevated APC activity.