Editing Helicobacter pylori (section)

===Transcriptome===
[[Single-cell transcriptomics]] using [[Single-cell transcriptomics#Single-cell RNA-seq|single-cell RNA-Seq]] gave the complete [[transcriptome]] of ''H. pylori'' which was published in 2010. This analysis of its [[Bacterial transcription|transcription]] confirmed the known acid induction of major [[Virulence factor|virulence]] loci, including the urease (ure) operon and the Cag [[pathogenicity island]] (PAI).<ref name="Sharma2010">{{cite journal | vauthors = Sharma CM, Hoffmann S, Darfeuille F, Reignier J, Findeiss S, Sittka A, Chabas S, Reiche K, Hackermüller J, Reinhardt R, Stadler PF, Vogel J | title = The primary transcriptome of the major human pathogen Helicobacter pylori | journal = Nature | volume = 464 | issue = 7286 | pages = 250–5 | date = March 2010 | pmid = 20164839 | doi = 10.1038/nature08756 | bibcode = 2010Natur.464..250S | s2cid = 205219639 }}</ref> A total of 1,907&nbsp;[[transcription start site]]s 337&nbsp;primary [[operon]]s, and 126&nbsp;additional suboperons, and 66&nbsp;mono[[cistron]]s were identified. Until 2010, only about 55&nbsp;transcription start sites (TSSs) were known in this species. 27% of the primary TSSs are also antisense TSSs, indicating that – similar to ''[[Escherichia coli|E.&nbsp;coli]]'' – [[Antisense RNA|antisense transcription]] occurs across the entire ''H.&nbsp;pylori'' genome. At least one antisense TSS is associated with about 46% of all [[open reading frame]]s, including many [[housekeeping gene]]s.<ref name="Sharma2010"/> About 50% of the [[Five prime untranslated region|5{{prime}} UTRs]] (leader sequences) are 20–40&nbsp;nucleotides (nt) in length and support the AAGGag motif located about 6&nbsp;nt (median distance) upstream of start codons as the consensus [[Shine-Dalgarno sequence|Shine–Dalgarno sequence]] in ''H.&nbsp;pylori''.<ref name="Sharma2010"/>