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Hydrophobic effect
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== Protein purification == In [[biochemistry]], the hydrophobic effect can be used to separate mixtures of proteins based on their hydrophobicity. [[Column chromatography]] with a hydrophobic stationary phase such as [[phenyl]]-[[sepharose]] will cause more hydrophobic proteins to travel more slowly, while less hydrophobic ones [[elute]] from the column sooner. To achieve better separation, a salt may be added (higher concentrations of salt increase the hydrophobic effect) and its concentration decreased as the separation progresses.<ref>{{Cite book|title=Protein Purification|last=Ahmad|first=Rizwan|publisher=InTech|year=2012|isbn=978-953-307-831-1}}</ref>
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