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Paper chromatography
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==Types== [[File:T.baccata-paper chromatography.jpg|thumb|80px|''[[Taxus baccata]]'' paper chromatography.]] ===Descending=== Development of the chromatogram is done by allowing the solvent to travel down the paper. Here, the mobile phase is placed in a solvent holder at the top. The spot is kept at the top and solvent flows down the paper from above. ===Ascending=== Here the solvent travels up the chromatographic paper. Both descending and ascending paper chromatography are used for the separation of organic and inorganic substances. The sample and solvent move upward. ===The ascending and descending method=== This is the hybrid of both of the above techniques. The upper part of ascending chromatography can be folded over a rod in order to allow the paper to become descending after crossing the rod. ===Circular chromatography=== A circular filter paper is taken and the sample is deposited at the center of the paper. After drying the spot, the filter paper is tied horizontally on a [[Petri dish]] containing solvent, so that the wick of the paper is dipped in the solvent. The solvent rises through the wick and the components are separated into concentric rings. ===Two-dimensional=== In this technique a square or rectangular paper is used. Here the sample is applied to one of the corners and development is performed at a right angle to the direction of the first run.
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