Editing DNA sequencing (section)

==== Polony sequencing ====
{{Main|Polony sequencing}}
The [[polony sequencing]] method, developed in the laboratory of [[George M. Church]] at Harvard, was among the first high-throughput sequencing systems and was used to sequence a full ''[[E. coli]]'' genome in 2005.<ref name=Shendure2005>{{cite journal | vauthors = Shendure J, Porreca GJ, Reppas NB, Lin X, McCutcheon JP, Rosenbaum AM, Wang MD, Zhang K, Mitra RD, Church GM | title = Accurate multiplex polony sequencing of an evolved bacterial genome. | journal = Science | volume = 309 | issue = 5741 | pages = 1728–32 | date = 9 September 2005 | pmid = 16081699 | doi = 10.1126/science.1117389 | bibcode = 2005Sci...309.1728S | s2cid = 11405973 | doi-access = free }}</ref> It combined an in vitro paired-tag library with emulsion PCR, an automated microscope, and ligation-based sequencing chemistry to sequence an ''E. coli'' genome at an accuracy of >99.9999% and a cost approximately 1/9 that of Sanger sequencing.<ref name=Shendure2005 /> The technology was licensed to Agencourt Biosciences, subsequently spun out into Agencourt Personal Genomics, and eventually incorporated into the [[Applied Biosystems]] SOLiD platform. Applied Biosystems was later acquired by [[Life Technologies (Thermo Fisher Scientific)|Life Technologies]], now part of [[Thermo Fisher Scientific]].