Editing Protein engineering (section)

====Sequence saturation mutagenesis (SeSaM)====
[[Sequence saturation mutagenesis]] results in the randomization of the target sequence at every nucleotide position. This method begins with the generation of variable length DNA fragments tailed with universal bases via the use of template transferases at the 3' termini. Next, these fragments are extended to full length using a single stranded template. The universal bases are replaced with a random standard base, causing mutations. There are several modified versions of this method such as SeSAM-Tv-II, SeSAM-Tv+, and SeSAM-III.<ref name=PoluriBook/>{{page needed|date=May 2017}}