Editing Candida albicans (section)

===CIp10 integrative plasmid===

Contrary to the yeast [[Saccharomyces cerevisiae|''S. cerevisiae'']] episomal plasmids do not stay stable in ''C. albicans''. In order to work with plasmids in ''C. albicans'' an integrative approach (plasmid integration into the genome) thus has to be used. A second problem is that most plasmid transformations are rather inefficient in ''C. albicans''; however, the CIp10 plasmid overcomes these problems and can be used with ease to transform ''C. albicans'' in a very efficient way. The plasmid integrates inside the RP10 locus as disruption of one RP10 allele does not seem to affect the viability and growth of ''C. albicans''. Several adaptations of this plasmid have been made after the original became available.<ref>{{cite journal | vauthors = Chauvel M, Nesseir A, Cabral V, Znaidi S, Goyard S, Bachellier-Bassi S, Firon A, Legrand M, Diogo D, Naulleau C, Rossignol T, d'Enfert C | display-authors = 6 | title = A versatile overexpression strategy in the pathogenic yeast Candida albicans: identification of regulators of morphogenesis and fitness | journal = PLOS ONE | volume = 7 | issue = 9 | pages = e45912 | year = 2012 | pmid = 23049891 | pmc = 3457969 | doi = 10.1371/journal.pone.0045912 | doi-access = free | bibcode = 2012PLoSO...745912C }}</ref><ref name="auto2">{{cite journal | vauthors = Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ | title = Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney | journal = Fungal Genetics and Biology | volume = 46 | issue = 2 | pages = 210–219 | date = February 2009 | pmid = 19032986 | pmc = 2698078 | doi = 10.1016/j.fgb.2008.10.012 }}</ref>