Editing Candida albicans (section)

===Candida two-hybrid (C2H) system===

Due to the aberrant codon usage of ''C. albicans'' it is less feasible to use the common host organism (''[[Saccharomyces cerevisiae]]'') for [[two-hybrid screening|two-hybrid studies]]. To overcome this problem a ''C. albicans'' two-hybrid (C2H) system was created. The strain SN152 that is auxotrophic for leucine, arginine and histidine was used to create this C2H system. It was adapted by integrating a HIS1 reporter gene preceded by five LexAOp sequences.
In the C2H system the bait plasmid (pC2HB) contains the ''[[Staphylococcus aureus]]'' LexA BD, while the prey plasmid (pC2HP) harbors the viral AD VP16. Both plasmids are integrative plasmids since episomal plasmids do not stay stable in ''C. albicans''. The reporter gene used in the system is the ''HIS1'' gene. When proteins interact, the cells will be able to grow on medium lacking histidine due to the activation of the ''HIS1'' reporter gene.<ref name="Calderone"/><ref name="stynen"/> Several interactions have thus far been detected using this system in a low scale set up.<ref name="stynen"/><ref>{{cite journal | vauthors = Legrand M, Bachellier-Bassi S, Lee KK, Chaudhari Y, Tournu H, Arbogast L, Boyer H, Chauvel M, Cabral V, Maufrais C, Nesseir A, Maslanka I, Permal E, Rossignol T, Walker LA, Zeidler U, Znaidi S, Schoeters F, Majgier C, Julien RA, Ma L, Tichit M, Bouchier C, Van Dijck P, Munro CA, d'Enfert C | display-authors = 6 | title = Generating genomic platforms to study Candida albicans pathogenesis | journal = Nucleic Acids Research | volume = 46 | issue = 14 | pages = 6935–6949 | date = August 2018 | pmid = 29982705 | pmc = 6101633 | doi = 10.1093/nar/gky594 }}</ref> A first high-throughput screening has also been performed.<ref>{{cite journal | vauthors = Schoeters F, Munro CA, d'Enfert C, Van Dijck P | title = A High-Throughput ''Candida albicans'' Two-Hybrid System | journal = mSphere | volume = 3 | issue = 4 | date = August 2018 | pmid = 30135223 | pmc = 6106057 | doi = 10.1128/mSphere.00391-18 }}</ref><ref name="frontiersin.org">{{cite journal | vauthors = Schoeters F, Van Dijck P | title = Protein-Protein Interactions in ''Candida albicans'' | journal = Frontiers in Microbiology | volume = 10 | pages = 1792 | date = 2019 | pmid = 31440220 | pmc = 6693483 | doi = 10.3389/fmicb.2019.01792 | doi-access = free }}</ref> Interacting proteins can be found at the [[BioGRID]].<ref>{{cite web|url=https://thebiogrid.org/|title=BioGRID - Database of Protein, Chemical, and Genetic Interactions|vauthors=Tyers M|website=thebiogrid.org|access-date=2018-08-25|archive-date=2017-09-11|archive-url=https://web.archive.org/web/20170911015109/https://thebiogrid.org/|url-status=live}}</ref>