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Protein engineering
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====Mega primed and ligase free focused mutagenesis==== This site saturation mutagenic technique begins with one mutagenic oligonucleotide and one universal flanking primer. These two reactants are used for an initial PCR cycle. Products from this first PCR cycle are used as mega primers for the next PCR.<ref name=PoluriBook/>{{page needed|date=May 2017}}
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