Editing Antisense RNA (section)

=== Cis-acting ===
{{See also|Cis-natural antisense transcript}}
[[Cis-acting]] asRNAs are transcribed from the opposite strand of the target gene at the target gene locus. They often show high degree or complete complementarity with the target gene. If the cis-acting asRNA regulates gene expression by targeting mRNA, it can only target individual mRNA. Upon interactions with the targeting mRNAs, cis-acting asRNAs can either block ribosome binding or recruit [[Ribonuclease|RNAase]] to degrade the targeting mRNAs. Consequently, the function of these cis-acting asRNAs is to repress translation of the targeting mRNAs.<ref name="Saberi_2016" /> Besides cis-acting asRNAs that target mRNAs, there are cis-acting epigenetic [[Silencer (genetics)|silencers]] and [[Activator (genetics)|activators]]. Antisense RNA has been shown to repress the translation of LINE1-ORF2 domain of [[Entamoeba histolytica]]. However it is not confirmed yet whether its cis-acting or trans.<ref>{{cite journal|doi=10.1016/j.plasmid.2021.102560|pmid=33482228|title=Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain|year=2021|last1=Kaur|first1=Devinder|last2=Agrahari|first2=Mridula|last3=Singh|first3=Shashi Shekhar|last4=Mandal|first4=Prabhat Kumar|last5=Bhattacharya|first5=Alok|last6=Bhattacharya|first6=Sudha|journal=Plasmid|volume=114|page=102560}}</ref>

In terms of epigenetic modification, cis-acting refers to the nature of these asRNAs that regulate epigenetic changes around the [[Locus (genetics)|loci]]  where they are transcribed. Instead of targeting individual mRNAs, these cis-acting epigenetic regulators can recruit chromatin modifying enzymes which can exert effects on both the transcription loci and the neighboring genes.<ref name="Magistri_2012" />