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Assay
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===Time and number of measurements taken=== Depending on whether an assay just looks at a single time point or timed readings taken at multiple time points, an assay may be: #An '''end point assay''', in which a single measurement is performed after a fixed incubation period; or #A '''kinetic assay''', in which measurements are performed multiple times over a fixed time interval. Kinetic assay results may be visualized numerically (for example, as a slope parameter representing the rate of signal change over time), or graphically (for example, as a plot of the signal measured at each time point). For kinetic assays, both the magnitude and shape of the measured response over time provide important information. #A '''high throughput assay''' can be either an endpoint or a kinetic assay usually done on an automated platform in 96-, 384- or 1536-well microplate formats ('''High Throughput Screening'''). Such assays are able to test large number of compounds or analytes or make functional biological readouts in response to a stimuli and/or compounds being tested.<ref>{{cite web |url=https://www.ncbi.nlm.nih.gov/books/NBK343428/ |title=Assay Guidance Manual [Internet] |last1=Sittampalam |first1= GS|date=2004 |website=ncbi.nlm.com |publisher=Eli Lilly & Company and the [[National Center for Advancing Translational Sciences]] |access-date= 12 Aug 2016}}</ref>
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