Editing Autoradiograph (section)

===Detection of protein phosphorylation===

[[Phosphorylation]] means the posttranslational addition of a [[phosphate group]] to specific amino acids of proteins, and such modification can lead to a drastic change in the stability or the function of a protein in the cell. Protein phosphorylation can be detected on an autoradiograph, after incubating the protein in vitro with the appropriate [[kinase]] and γ-32P-ATP. The radiolabeled phosphate of latter is incorporated into the protein which is isolated via [[SDS-PAGE]] and visualized on an autoradiograph of the gel. (See figure 3. of a recent study showing that [[CREB-binding protein]] is phosphorylated by [[HIPK2]].<ref name="pmid26247811">{{cite journal | vauthors = Kovacs KA, Steinmann M, Halfon O, Magistretti PJ, Cardinaux JR | title = Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2 | journal = Cell Signaling | volume = 27 | issue = 11 | pages = 2252–60 | date = Nov 2015 | pmid = 26247811 | doi = 10.1016/j.cellsig.2015.08.001 | url = https://repository.ist.ac.at/578/1/CLS-D-15-00072R1_.pdf }}</ref>)