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CDNA library
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== cDNA Library uses == cDNA libraries are commonly used when reproducing eukaryotic genomes, as the amount of information is reduced to remove the large numbers of non-coding regions from the library. cDNA libraries are used to express eukaryotic genes in prokaryotes. Prokaryotes do not have introns in their DNA and therefore do not possess any enzymes that can cut it out during transcription process. cDNA does not have introns and therefore can be expressed in prokaryotic cells. cDNA libraries are most useful in [[reverse genetics]] where the additional genomic information is of less use. Additionally, cDNA libraries are frequently used in [[functional cloning]] to identify genes based on the encoded protein's function. When studying eukaryotic DNA, expression libraries are constructed using complementary DNA (cDNA) to help ensure the insert is truly a gene.<ref name=":1">{{Cite book|title=Biotechnology : applying the genetic revolution|last=P.|first=Clark, David|date=2009|publisher=Academic Press/Elsevier|others=Pazdernik, Nanette Jean.|isbn=9780121755522|location=Amsterdam|oclc=226038060}}</ref> === cDNA Library vs. Genomic DNA Library === cDNA library lacks the non-coding and regulatory elements found in genomic DNA. [[Genomic DNA library|Genomic DNA libraries]] provide more detailed information about the organism, but are more resource-intensive to generate and keep.
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